Lady Gaga will make her acting debut in filmmaker Robert Rodriguez’s sequel to 2010’s Machete. Like Machete, Machete Kills will star Danny Trejo as Machete Cortez. He will be backed by a cast that includes Mel Gibson, Charlie Sheen, Jessica Alba, Amber Heard, Michelle Rodriguez and Cuba Gooding Jr., among others. Lady Gaga will play the role of La Cameleón, which appears to be the counterpart of Gooding Jr.’s role as El Cameleón. Catch the two glimpses of Gaga at the 1:21 and 1:35 mark of the trailer above and view her poster for the film below. Machete Kills arrives in theaters October 4.